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01/02
Molecular Diagnostics

What is PCR and how does it work?

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ANSWER

Polymerase Chain Reaction amplifies specific DNA segments. Three steps repeated 25-35 cycles: 1. Denaturation (94°C): DNA strands separate 2. Annealing (50-65°C): primers bind to target sequences 3. Extension (72°C): Taq polymerase synthesizes new strands. Each cycle doubles the DNA, producing millions of copies from a tiny sample. Essential for diagnostics, forensics, and research.

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